At the Laboratory of Dental Regenerative Medicine, we restore the function of pulp tissue of teeth with deep cavities or cavities extends to nerves (pulp) and tooth with accumulated pus under the roots forming periapical lesion. We are conducting research on pulp regeneration treatment methods that will have longevity effect for patients.
Currently, it is known that stem cells exist in the bone marrow in bone and many other tissues in the body. The pulp tissue in the tooth contains a lot of stem cells, which are especially beneficial for regenerations blood vessels and nerves, and it is recognized to remove discarded teeth that are not related to occlusion (for convenience with wisdom teeth or orthodontic treatment). Pulp stem cells can be removed from those teeth and can be used for further treatment. Recently, human stem cells have been introduced regarding pulp regeneration treatment method in which pulp stem cells taken out from a patient's own discarded tooth and transplanted into the root canal after the pulp has been removed to restore the functioning pulp of the tooth. This clinical research has been conducted since April 2013 with the approval of the Minister of Health, Labor and Welfare based on the "Guidelines for Clinical Research Used". As a result, it was confirmed that there was no problem with safety in 4 out of 5 cases and the sensitivity of the dental nerve returned within 1 month after the treatment, suggesting that this treatment method was effective.
Laboratory of Dental Regenerative Medicine is developing new treatments for dental caries and pulpitis.
This research was adopted as one of 24 national projects to promote the development and practical application of advanced medical care as a super special zone in December 2008.
One of the techniques is in the case of "deep dental caries treatment", the dentin is restored by dentin regeneration.
Next, in the case of "treatment of pulpitis in which a part of the pulp is damaged", the pulp function was restored by applying MMP3, which is a kind of enzyme, found on the surface of the pulp to suppress inflammation and regenerate the pulp and even dentin.
Thirdly, in the case of "pulp removal treatment in which pulpitis is severe and the entire pulp must be removed", or in the case of "infected root canal treatment in which pus accumulates under the root", the inner portion of the root (root canal) is cleaned and disinfected. After that, pulp regeneration was done by transplanting "own pulp stem cells" and "cell migration factor" together with "atelocollagen". In the root canal, blood vessels, nerves and pulp are regenerated in a short period of time, and the dentin lid is regeneration at the top, allowing it to return to a living healthy tooth.
Fourth, it is becoming clear that the nanobubble drug introduction method can completely kill bacteria that have invaded deep into the roots of infected root canal teeth in a short period of time. Currently, we are developing a nanobubble generator so that it can be applied to humans.
In particular, regarding the third point of tooth pulp regeneration technology, as an important issue set by the National Council for Science and Technology Policy. In 2010, the "Research and Development System for Accelerating the Practical Use of Health Research Results" of the science and technology strategy promotion, we received the support of experts for the safety and efficacy evaluation of clinical research through the "Program to Support the Elimination of Conflicts". In November 2012, we got approval from the Minister of Health, Labor and Welfare to carry out clinical research on human stem cells, and from April 2013, we conducted 5 clinical studies, and the safety was confirmed and the effectiveness was suggested.
With these technologies, the amount of caries scraped can be minimized and the functions of pulp and dentin can be restored.
In addition, there is no gap for bacteria to invade from the mouth, and it also has a mechanism to prevent tooth decay again. Therefore, it seems to be a technology that helps to make teeth last longer. The technology to produce a large amount of tubule dentin in a short period of time, a drug for treating pulpitis, and the complete regeneration of pulp have not been reported so far, so that it has an international advantage.
The National Center for Geriatrics and Gerontology has a mission to promote the independence of the mind and body of the elderly and contribute to the construction of a healthy and longevity society.
Based on that philosophy, Laboratory of Dental Regenerative Medicine conducts innovative dental medicine and its development research aimed at preserving and prolonging the life of teeth.
Specifically, in dental pulpitis or apical periodontitis *, clinical research aimed at "restoring teeth and completely recovering their functions by the world's first new pulp and dentin regeneration treatment method". We performed 5 cases, confirmed the safety, and suggested the effectiveness. This treatment method can be fully applied to middle-aged and elderly people for maintaining the dental health of the elderly. It leads to maintenance of masticatory / communication function, prevention of dementia, maintenance of motor function, etc. We believe that we can contribute to the improvement of living functions of the elderly.
With the cooperation of companies to achieve these goals, the staff of the Research Department will work together on a daily basis with the following goals so that we can deliver dental regenerative medicine to all the people as soon as possible.
Iohara K, Tominaga M, Watanabe H, Nakashima M. Periapical bacterial disinfection is critical for dental pulp regenerative cell therapy in apical periodontitis in dogs. Stem Cell Res Ther. 2024 Jan 17; 15(1):17. doi: 10.1186/s13287-023-03628-6
Sakatoku S, Hayashi Y, Futenma T, Sugita Y, Ishizaka R, Nawa H, and Iohara K. Periostin Is a Candidate Regulator of the Host Microenvironment in Regeneration of Pulp and Dentin Complex and Periodontal Ligament in Transplantation with Stem Cell-Conditioned Medium. Stem Cells International. 2024 Feb 23;2024:7685280. doi: 10.1155/2024/7685280. eCollection 2024.PMID: 38435089
Zayed MH, Iohara K.Age Related Senescence, Apoptosis, and Inflammation Profiles in Periodontal Ligament Cells from Canine Teeth. Curr Mol Med. 2022 May 20. doi: 10.2174/1566524022666220520124630.
Nakashima M, Fukuyama F, Iohara K. Pulp Regenerative Cell Therapy for Mature Molars: A Report of 2 Cases. J Endod. 2022 Aug 5；S0099-2399(22)00510-6. doi: 10.1016/j.joen.2022.07.010. Online ahead of print.
Ziauddin SM, Nakashima M, Watanabe H, Tominaga M, Iohara K. Biological characteristics and pulp regeneration potential of stem cells from canine deciduous teeth compared with those
Zayed MH,Iohara K,Watanabe H,Ishikawa M,Tominaga M,Nakahima M Characterization of stable hypoxia-preconditioned dental pulp stem cells compared with mobilized dental pulp stem cells for application for pulp regenerative therapy. Stem Cell Res Ther, 12(1):302, 2021
Nakahima M,Iohara K,Zayed M Pulp Regeneration: Current Approaches, Challenges, and Novel Rejuvenating Strategies for an Aging Population. J Endodontics. 2020;46(9S):S135-S142.
Iohara K,Nakahima M Examination of root canal sterilization effect by drug-containing nanobubble water in intractable apical periodontal disease. 63 (1): 73-82, 2020.
Iohara K,Zayed M,Takei M,Watanabe H,Nakahima M Treatment of Pulpectomized Teeth with Trypsin prior to Transplantation of Mobilized Dental Pulp Stem Cells Enhances Pulp Regeneration in Aged Dogs. Frontiers Bioengineering. 14 August 2020. doi.org/10.3389/fbioe.2020.00983
Zayed MH,Iohara K,Nakashima M Antagonist of CCR3 stimulates pulp regeneration by transplantation of mobilized dental pulp stem cells in aged dog teeth. Scientific Reports. 10, Article number: 8631 (2020)
Azim AA,Iohara K anobubble-Enhanced Antimicrobial Agents: A Promising approach For Regenerative Endodontics. The Journal of Endodontics. 2020 Sep; 46 (9): 1248-1255. Doi: 10.1016 / j.joen.2020.06.002 . Epub 2020 Jun 15.
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